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NxGen™ phi29 DNA Polymerase

  • Excellent strand displacement, fidelity and speed.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
NxGen™ phi29 DNA Polymerase  2,000 U 30221-1 $99.00
   10,000 U 30221-2 $399.00
Pyrophosphatase Inorganic, Thermostable  250 Units 30074-1 $64.00
   (5 x 250 U) 30074-2 $255.00
ORDER INFORMATION

Supplied at 10,000 U/mL in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C. Also supplied is 10X phi29 DNA Polymerase Buffer composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25°C. 10,000 U is supplied as 5 × 2,000 U.

This product is sold for in vitro research use only under license from Brookhaven National Laboratory, U.S. Patent Nos. 4,952,496; 5,693,489; and 5,869,320.

Description

NxGen Enzymes Iconphi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

 

phi29 Price Comparison
An example of the savings you can expect.

Specifications

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.

Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.

Unit Concentration 10,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease Functional
E. coli 16S rDNA Contamination 100 U <10 copies
Endonuclease 100 U <10% converted
Storage -20&°C

Resources

Document File Name Type
Manual MA114-phi29 PDF
Product Flyer NexGen-Enzymes-2011 PDF
PDF MSDS PDF

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