CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC): pSMART BAC
- Efficiently clone any size insert up to 150kb+.
- Create libraries from even A/T- or G/C-rich genomes.
- Clone gene clusters or operons.
- High insert stability.
- Inducible copy number.
Ordering
Each CopyRight® v2.0 BAC Cloning Kit contains: pre-cut vector (pSMART BAC with a choice of BamHI or EcoRI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, Arabinose Induction Solution, and complete protocols. Each CopyRight pEZ BAC Cloning Kit contains: pre-cut CopyRight vector (pEZ BAC BamHI or pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>2 x 1010 cfu/µg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.
Description
Lucigen's CopyRight® v2.0 BAC cloning kits with pSMART® BAC (Figure1) or pEZ BAC (Figure 2) vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented accuracy, reliability, and efficiency in BAC cloning and library construction. These vectors combine the revolutionary, patented CloneSmart® transcription-free technology which minimizes cloning bias and enables cloning of otherwise toxic genes, inducible copy number amplification (20-50X) for high DNA yields, and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants resulting in zero background. Unlike other BAC cloning systems, Lucigen's CloneSmart technology assures that even "unclonable" inserts are stably maintained; sequences are not lost or rearranged in the cloning process. CopyRight Kits offer superior performance for BAC cloning at a much better price than other available kits. Figure 3 shows an example of a Random Shear BAC library generated with pSMART BAC.
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Figure 1. Diagram of CopyRight vector pSMART BAC. Positions of ori2, repE, IncC, origin of replication (single copy); oriV, inducible origin of replication; par A,B,C, partition genes; Cm^r, chloramphenicol-resistance gene; cosN - lambda packaging signal; T, CloneSmart transcription terminators; sacB, sucrase gene; lacZ, alpha peptide portion of the beta galactosidase gene. Approximate positions of sequencing primers and transcription terminators are indicated. |
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Figure 2. Diagram of the CopyRight vector pEZ BAC. Positions of ori2, repE, IncC, origin of replication (single copy); oriV, inducible origin of replication; par A,B,C, partition genes; Cm^r, chloramphenicol-resistance gene; cosN, lambda packaging signal. Approximate positions of sequencing primers and transcription terminators are indicated. |
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Figure 3. Arabidopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained with an average insert size of 105 kb and ~5X genome coverage. |
Convenient Success
Efficiently obtain your clone or create your library—the CopyRight v2.0 Cloning Kits eliminate tedious vector and competent cell preparation, as well as time-consuming QC testing experiments. Kits include optimized reagents, ligation-ready vector, highly efficient electrocompetent cells, detailed instructions, and trouble-shooting guide to simplify cloning and sequencing.
Resources
| Document | File Name | Type |
| Manual | MA028-CopyRight-v2.0-BAC-Cloning-Kits | |
| MSDS |
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