pRANGER™-BTB

Broad host range expression cloning
The pRANGER-BTB vectors [Lynch, M.D. and Gill, R.T. (2006). Biotechnol. Bioeng., 94 (1) 151-158] provide high stability cloning in a broad host-range expression vector. These unique vectors incorporate the pBBR1 replicon, which enables stable plasmid replication in Gram negative bacteria, and the pBAD promoter for arabinose-inducible gene expression. These features allow cloning and expression in many Gram negative strains. The pRANGER-BTB vectors are available with ampicillin, kanamycin, chloramphenicol, or trimethoprim resistance (Figure 1).

Figure 1. pRANGER-BTB core vector. pBAD - arabinose-inducible promoter; araC - araC gene for arabinose induction; rep - pBBR1 origin of replication; MCS - multiple cloning site (EcoRI, HincII, HindIII); DrugR - drug resistance gene. The pRANGER-BTB vectors differ only in the DrugR gene: ampicillin, kanamycin, chloramphenicol, or trimethoprim resistance. Approximate locations of transcription terminators (T) are indicated. pRANGER-BTB vectors have a low copy number (~ 30 per cell).