Modifying Enzymes Selection Guide

NxGen™ Genomic Grade Enzymes

Exceptional Performance for Demanding Applications.

Lucigen's NxGen™ enzymes are ideally suited to Next-Gen applications such as sequencing & array analysis that require effective and pure reagents. Achieve optimal results even in highly-sensitive applications.

Industry-leading value and quality.
99% purity for consistency and reliability.
Convenient sizing for ease of use and ordering.

Molecular Biology Grade Enzymes

Enzymes for Molecular Biology Applications.

A collection of enzymes used to create or modify RNA/DNA samples, allowing you to easily perform:

Labeling or RT-PCR for cloning, sequencing or detection.
Phosphate addition or removal.
RNA Degradation.

Polymerases

Powerful enzymes to provide accurate generation of nucleic acid polymers from a wide range of starting molecules. Also see PCR & Amplification Enzymes

	Applications	Proofreading	Strand Displacement	Error Rate	Thermal Stability	Extend from RNA Primer	Extend from Nick
phi29 Polymerase	♦	3´→5´ Strong	Very strong			✓	✓
M-MuLV Reverse Transcriptase	cDNA synthesis ♠		Strong
T7 RNA Polymerase	Transcription, Gene Expression
T7 DNA Polymerase	Site-directed mutagenesis ♦	3´→5´ Very strong		15 × 10^-6		✓
Terminal Transferase	3' Labeling
Bst DNA Polymerase Amplification & PCR	♦❖		Very strong		✓
DNA Polymerase I Large (Klenow), Exonuclease minus	Labeling	3´→5´ Strong	Strong	100 × 10^-6		✓	✓
T4 DNA Polymerase	Fill-in, mutagenesis	3´→5´ Very strong		low		✓
T4 DNA Polymerase, Exonuclease minus	♦			low		✓

Applications: ♠ = RT-PCR ♦ = Sequencing ❖ = Cloning

Also available: Pyrophosphatase – accelerate your polymerase reactions.

Ligases

Enzymes that easily link together diverse or unmodified nucleic acid polymers, including nicked strands, blunt and cohesive ends, and heteroduplex formation.

	Applications	Ligate Cohesive Ends	Ligate Blunt Ends	Nicks in dsDNA	DNA-RNA Heteroduplex Formation	RNA 3' Labeling	ssRNA and dsRNA oligo joining
T4 DNA Ligase, Low Concentration	Cloning, Linker addition	✓	✓	✓	✓
T4 DNA Ligase, High Concentration Rapid Kit	Cloning, Linker addition	✓	✓	✓	✓
T4 RNA Ligase	RNA/DNA joining, 3' Labeling					✓	✓

Nucleases

Efficiently modify your single or double-stranded DNA in any condition to obtain modifications at either end.

		DNA compatibility						Partial ss Extension Digestsion
	Polarity	Substrate	Activity without 5'-phosphate	3' Recessed Ends	3' Pro-truding Ends	Blunt Ends	Nicks	Partial ss Extension Digestsion	Product	Phosphorothioate cleavage
Lambda Exonuclease	5´→3´	ssDNA, dsDNA	1		✓	✓		3'	ssDNA, dNMP
Exonuclease I	3´→5´	ssDNA	✓						dNMP3
Exonuclease III	3´→5´	ssDNA, dsDNA	✓	✓	2		✓	5'	ssDNA, dNMP
RNAse I		ssRNA							Ribonucleotides

1 = Strongly prefers 5'-P, but will degrade without it.
2 = Exo III cannot blunt a 3' extension.
3 = Exo I cannot cleave the final bond, yielding dNMP's and a dinucleotide.

Modifying Enzymes Selection Guide