Exclusive Linear Vector technology gets every gene and sequence
“TIGR had tried several times [to make a large-insert Tetrahymena library], but the largest size range we could obtain was 4-6 kb. Lucigen was able to provide a library in the pJAZZ® vector with an average insert size of 12 kb and some inserts as large as 20 kb.”
-- Dr. Robert Coyne, The Institute for Genome Research (TIGR)
- Libraries created from difficult genomes such as Flavobacterium columnare (69% A/T)
- Inserts up to 30kb in the linear pJAZZ® Vector.
Lucigen’s first-of-its-kind BigEasy® pJAZZ® linear cloning vectors represent a major advance in large insert library construction. Circular plasmid vectors become supercoiled in the host cell, causing torsional stress and insert instability. In contrast, pJAZZ vectors remain linear and torsion-free. Sequences that are unclonable in plasmid vectors are easily and stably cloned in pJAZZ. This superior insert stability allows the construction of much more accurate and complete libraries, from <1 kb to over 30 kb, that include the genes and sequences inevitably lost when using plasmid vectors. The pJAZZ vectors are simply the best choice for constructing large insert libraries from difficult-to-clone genomic DNAs, large cDNAs, and long PCR products (Figures 1 and 2).
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Fco-pJAZZ 6-10 kb
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Figure 1. F. columnare genomic library (6-10 kb inserts; ~70% AT) constructed in the pJAZZ vector. Plasmids were digested with NotI. M, 1 kb ladder. The 2.2 kb and 11.8 kb bands are the vector arms of pJAZZ. -Data courtesy of A. Karsi and M.L. Lawrence, Mississippi State University |
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| Figure 2. The F. columnare genome in the pJAZZ vector was sequenced to seven-fold coverage. Only 10 clone gaps were found in the 3.2 MB genome. |
What you get
Lucigen delivers bSMART large insert libraries as frozen clones in 96- or 384-well plates, with a complete report and guidelines on storing and working with your bSMART Library. Our goal is to successfully deliver all genes and DNA sequences to our clients at a highly competitive price.
