TaqSelect™ DNA Polymerase

Outstanding PCR performance

Lucigen offers its high quality Taq polymerase in a 2X Premix containing a stabilizer/enhancer optimized for performance. The high density reaction buffer contains magnesium at an optimal concentration for PCR and allows direct loading after thermocycling. TaqSelect DNA Polymerase offers the same high performance of EconoTaq PLUS 2x Master Mix, but does not contain dNTPs (deoxynucleotides).

Applications

• Routine PCR.
• Genotyping.
• Amplify challenging templates.
• Preparation of PCR fragments for cloning.

Please Note:
Some applications in which Lucigen’s TaqSelect DNA Polymerase can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

Composition. TaqSelect DNA Polymerase contains: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 3mM MgCl₂, 0.2 % Triton X-100, 100 U/ml Taq DNA Polymerase, and a proprietary PCR stabilizer/enhancer.

Enzyme Activity Determination. One unit of TaqSelect DNA Polymerase catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl₂, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.

Functional testing. TaqSelect DNA Polymerase is tested for performance in amplifying a 4 kb region from 40 ng of plasmid DNA, and a 2.7 kb region from 70 ng of genomic DNA. The resulting PCR products are visualized on an ethidium bromide-stained agarose gel.

Purity. TaqSelect DNA Polymerase is free of contaminating DNA, as well as endonuclease and nicking activities. The enzyme is greater than 99% pure by SDS-PAGE.

Absence of Endonuclease or Nicking Activity: Incubation of 10 U of TaqSelect DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of 10 U of TaqSelect DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.