“The TG1 cells I ordered worked great, and I really appreciate your efforts in getting me the cells in such a short time period. The cells have a super-high transformation efficiency [...] and I achieved a much bigger library than I was expecting (great news for me). Thanks, Wei, Pharmaceutical Researcher.

“Lucigen’s cells ROCK! Few, if any, companies or individual laboratories make cells with as high efficiencies.”
- Dr. Douglas Crawford, University of Miami, Miami, FL

"I have recently tried a sample pack of your Electrocompetent Cells for Phage Display (TG1, ER2738 and SS320). They worked absolutely great! I was quite impressed with the transformation efficiency. No other cells I've tested have come close to this! Sincerely, Dr Jason LeBlanc"
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Phage Display Competent Cells: TG1 Electrocompetent Cells

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available: ≥4 × 1010 cfu/µg.
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells

Ordering

Product Description Size Cat. No. List Price Quantity BUY
TG1 Electrocompetent Cells  12 rxns (DUOs) 60502-1 $150.00
   24 rxns (DUOs) 60502-2 $261.00
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

Description

  • TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.

  • SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.

  • ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.


Figure 1. Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification.

Table 1. Competent Cells for Phage Display libraries


    Cell Lines

    Transformation Efficiency
    (cfu/µg pUC DNA)

    Cloning Methylated DNA

    BAC, Cosmid Cloning

    Blue/White Screening

    TG1 Electrocompetent

    >4 × 1010

    YES

    NO

    YES
    IPTG induction required

    SS320 (MC1061 F') Electrocompetent

    >4× 1010

    YES

    NO

    YES
    IPTG induction required

    ER2738 Electrocompetent

    >2 × 1010

    YES

    NO

    YES
    IPTG induction required

Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells for detailed information.

Specifications

Genotype

  • TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
  • SS320 (MC1061F'): [F'proAB+lacIqlacZΔM15 Tn10 (tetr)] hsdR mcrB araD139 Δ(araABC-leu)7679ΔlacX74 galUgalK rpsL thi
  • ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5

Resources

Document File Name Type
Manual MA053-Phage-Display PDF
Poster Phage-Display-Protein-Society-2010 PDF
Product Flyer Competent-Cells-Phage-Display PDF
Product Flyer Competent-Cell-Comparison-2011 PDF
PDF MSDS PDF

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