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T4 DNA Polymerase

  • Recombinant T4 DNA Polymerase
  • Many applications including creating blunt-ends and in vitro mutagenesis

Ordering

Product Description Size Cat. No. List Price Quantity BUY
T4 DNA Polymerase  300 U 30081-1 $93.00
   1500 U 30081-3 $407.00
ORDER INFORMATION
Recombinant T4 DNA Polymerase is supplied at a concentration of 3,000U/ml in a storage buffer of 50% glycerol, 100 mM KPO4 (pH 6.5) and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.

Description

T4 DNA Polymerase is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer.  Additionally, the enzyme has a strong 3’→5’ exonuclease activity.

Source:
Purified from a strain of E. coli that carries a T4 DNA polymerase expression plasmid.

Applications:

  • Fill in of 5’ overhanging ends to create blunt-ends (1).
  • Exonuclease removal of 3’ overhanging to create blunt-ends (1).
  • in vitro mutagenesis (2)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

  • T4 DNA Polymerase is active in many buffers and can often be used directly in a restriction digest reaction.
  • T4 DNA Polymerase exhibits no strand displacement activity.

References:

  1. Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.10-3.5.12.
  2. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382. San Diego: Academic Press.

Resources

Document File Name Type
Manual MA071-T4-DNA-Polymerase PDF
PDF MSDS PDF

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