RNase I

RNase I is a native E. coli enzyme that converts single-strand RNA to mononucleoside 3' monophosphates with inherently broad specificity, cleaving between all dinucleotide pairs with essentially the same efficiency (1,2,3).

Applications:

• Removal of RNA from DNA preparations
• RNA structural studies (4)
• cDNA cloning (5)
• Mismatch detection (6)
• RNase protection assay

Heat inactivation: 70°C for 10 minutes.

Storage:
Enzyme and buffer should be stored at -20°C; do not store below -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

• Broader specificity than RNase A means more complete digestion of RNA.
• High activity; high purity.
• Easily inactivated by heating to 70°C for 10 minutes.
• Preference for single-strand RNA allows detection of duplex RNA, single base mismatches and RNA structure.

References:

1.  Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.

2. Meador, J. et al.  (1990) Eur. J. Biochem. 187, 549.

3. Meader, J. and Kennel, D. (1990) Gene 95, 1.

4. Goldrick, M.M. (2001) Hum Mutat 18(3), 190.

5. Seki, M. (1998) Plant J. 15, 707.

6. McPike, M.P., et. al (2002) Nucleic Acids Res. 30, 2825.