PCRTerminator® End Repair Kit
- Optimizes cloning of any PCR product into blunt vectors
- Obtain higher cloning efficiency
- Easy to use
Ordering
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The PCRTerminator End Repair Kit contains: PCRTerminator End Repair Enzyme Mix, End Repair Buffer, and a complete protocol.
The PCRTerminator End Repair Kit contains: PCRTerminator End Repair Enzyme Mix, End Repair Buffer, and a complete protocol.
Description
Polish the ends of PCR products
PCR products generated with non-proofreading polymerases such as Taq, Tth, or Tfl primarily have single 3´ G- or 3´ A-overhangs. These products can be treated with the PCRTerminator Kit for cloning into blunt vectors (e.g., Lucigen’s CloneSmart®, CopyRight®, or BigEasy® systems). The PCRTerminator Kit also adds 5´ phosphates to amplification products, which is essential for cloning PCR products from non-proofreading or proofreading polymerases (such as Pfu or Vent®) into dephosphorylated vectors.
Resources
| Document | File Name | Type |
| Manual | MA001-PCR-Terminator | |
| Citations | PCRTerminator® End Repair Kit - Citations | LINK |
| MSDS |
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