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NxGen™ Lambda Exonuclease

  • Modify diverse 5’ ends of dsDNA.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
NxGen™ Lambda Exonuclease  5,000 U 30261-1 $99.00
   25,000 U 30261-2 $399.00
ORDER INFORMATION

Supplied at 5,000 U/mL in 25 mM Tris-HCl, 50 mM NaCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25°C. Also supplied is 10X Lambda Exo Reaction buffer which in 1X form is composed of 67 mM Glycine, 2.5 mM MgCl2, pH 9.4 @ 25°C. 25,000 U is supplied as 5 × 5,000 U.

Description

NxGen Enzymes IconLambda Exonuclease selectively degrades the 5′-phosphorylated (but not 5'-hydroxyl) strand of dsDNA. It is a highly processive enzyme that does not initiate at nicks or gaps, though it will degrade a 5′-overhanging tail from dsDNA non-preferentially.

Price Comparison of Lambda Exonuclease

Specifications

Unit Definition: One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C.

Source: Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.

Unit Concentration 5,000 U/mL
Purity (SDS-PAGE) >99%
Endonuclease 1500 U <10% converted
E.coli 16S rDNA Contamination 1500 U <10 copies
Storage -20°C

Resources

Document File Name Type
Manual MA124-Lambda PDF
Product Flyer NexGen-Enzymes-2011 PDF
PDF MSDS PDF

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