NxGen™ Terminal Transferase
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Effective end modification and labeling.
Ordering
ORDER INFORMATION
Supplied at 20,000 U/mL in 50 mM KPO4, 100 mM NaCl, 1.0 mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.3 @ 25°C. Also provided is 2.5 mM CoCl2 and 10X Terminal Transferase Buffer which in 1x form is composed of 20 mM Tris –Acetate, 50 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM DTT, pH 7.9 @ 25°C. 15,000 U is supplied as 5 × 3,000 U.
Supplied at 20,000 U/mL in 50 mM KPO4, 100 mM NaCl, 1.0 mM DTT, 0.1mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.3 @ 25°C. Also provided is 2.5 mM CoCl2 and 10X Terminal Transferase Buffer which in 1x form is composed of 20 mM Tris –Acetate, 50 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM DTT, pH 7.9 @ 25°C. 15,000 U is supplied as 5 × 3,000 U.
Description
Terminal deoxynucleotidyl Transferase (TdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of ssDNA or dsDNA. 3' tailing can be induced with the addition of 1mM Co2+. This protein is sold as an N-terminal truncation of the terminal transferase gene attached to an N-terminal fusion tag.
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Specifications
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 1 nmol of dTTPs into acid insoluble material in 1 hour at 37°C.
Source: An E. coli strain that carries the cloned terminal transferase gene from calf thymus.
| Unit Concentration | 20,000 U/mL |
| Purity (SDS-PAGE) | >99% |
| SS Exonuclease 200 U | <5.0% released |
| DS Exonuclease | 200 U <0.5% released |
| Endonuclease | 200 U <10% converted |
| E.coli 16S rDNA Contamination | 200 U <10 copies |
| Storage | -20°C |
Resources
| Document | File Name | Type |
| Manual | MA118-TdT | |
| Product Flyer | NexGen-Enzymes-2011 | |
| MSDS |
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