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NxGen™ T7 RNA Polymerase

  • Reliable transcription.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
NxGen™ T7 RNA Polymerase  25,000 U 30223-1 $99.00
   125,000 U 30223-2 $399.00
Pyrophosphatase Inorganic, Thermostable  250 Units 30074-1 $64.00
   (5 x 250 U) 30074-2 $255.00
NxGen™ RNAse Inhibitor  10,000 U 30281-1 $99.00
   50,000 U 30281-2 $399.00
ORDER INFORMATION
Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C. 125,000 U is supplied as 5 × 25,000 U.

Description

NxGen Enzymes IconT7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.

T7 RNA Polymerase

Specifications

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.

Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Unit Concentration 50,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease 500 U <0.1% released
DS Exonuclease 500 U <0.1% released
Endonuclease 500 U <10% converted
RNAse Contamination 500 U = none detected
E. coli 16S rDNA Contamination 500 U <10 copies
Storage -20°C

Resources

Document File Name Type
Manual MA117-T7-RNAP PDF
Product Flyer NexGen-Enzymes-2011 PDF
PDF MSDS PDF

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