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NxGen™ Exonuclease I

  • Remove nucleotides from the 3’ end of ssDNA.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
NxGen™ Exonuclease I   15,000 U 30262-1 $99.00
   75,000 U 30262-2 $399.00
ORDER INFORMATION

Supplied at 20,000 U/mL in 10 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 0.5 mM EDTA, 50% glycerol, pH 7.5 @ 25°C. Also provided is Exonuclease I Buffer, which in 1X form is composed of 67 mM Glycine, 6.7 mM MgCl2, 10 mM β-mercaptoethanol, pH 9.5 @ 25°C. 75,000 U is supplied as 5 × 15,000 U.

This product is sold for in vitro research use only under license from Brookhaven National Laboratory, U.S. Patent Nos. 4,952,496; 5,693,489; and 5,869,320.

Description

NxGen Enzymes IconExonuclease I digests ssDNA in the 3′→ 5′ direction, leaving ds DNA molecules and the 5'-terminus intact. Digestion is inhibited by 3′ -terminal phosphates. The enzyme can work in a range of buffer conditions and can usually tolerate buffers containing magnesium.

Price Comparison Exonuclease I

Specifications

Unit Definition: One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.

Source: Purified from a strain of E. coli that expresses the recombinant Exonuclease I gene.

Unit Concentration

20,000 U/mL

Purity (SDS-PAGE)

>99%

Endonuclease

200 U <10% converted

E.coli 16S rDNA Contamination

200 U <10 copies

Storage

-20°C

Resources

Document File Name Type
Manual MA122-Exo-I PDF
Product Flyer NexGen-Enzymes-2011 PDF
PDF MSDS PDF

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