High Molecular Weight genomic and BAC DNA preparation

• Genomic DNA up to Megabase-size provided from any source.
• DNA quality suitable for any application.

• BAC/Fosmid Libraries
  • Random Shear BAC
  • Novel (large insert) Fosmid
  • Partial Digestion BAC
• Sequencing
  • NextGen Sequencing
  • BAC end Sequencing
  • BAC Shotgun Sequencing
  • Sanger DNA Sequencing
• Other Libraries/Cloning
  • Difficult Genome
  • Large/Difficult Insert Cloning
  • NanoClone ™ & GapFree Shotgun
  • Metagenomic
• Library Screening & Tools
  • Pooling & Superpooling
  • Gridding & Colony Filters
  • Colony Preparation
  • Library Screening
• DNA Prep
  • BAC Miniprep / DNA Prep
  • HMW Genomic DNA Prep

Ultra pure High Molecular Weight genomic DNA

Proprietary techniques result in High Molecular Weight genomic DNA from:

• Microbes
• Plants
• Animals
• Any species
• Any tissue or cell
• BAC clones

DNA is prepared in solution or agarose plugs (optional) to ultra-high quality standards and is ready for any research use including,

• Clone-free library construction for Next-Gen sequencing
• Large-insert cloning in pSMART^® BAC or pJAZZ^® Vectors.
• Other cloning, PCR, etc.

What you get
At the completion of the project, you are provided ultrapure genomic DNA up to Megabase-size provided in TE buffer (chromosome-size for agarose DNA plugs) ready for your next application.  A complete report of quality testing is provided with each sample.

BAC DNA preparation

Ultra-high quality BAC DNA prepared in the high quantities needed for:

• Subcloning, constructing clone-free libraries for Next-gen sequencing, etc. from individual BAC clones
• BAC DNA pooling–can be customized to fit your needs
• Sanger BAC end sequencing. BAC End sequencing.*

*With Lucigen’s CopyRight BAC cloning system, sufficient DNA for forward and reverse reactions with up to 95% success and reads >800bp (Figure 2) can be produced.

Figure 2. Example of a BAC end sequence with read length over 800 bp.

Purified BAC large fragments

Large DNA fragments produced from BAC DNA or BAC clones.  Careful control of quantity and quality of DNA as well as optimization of digestion conditions is followed by CHEFF gel electrophoresis.  The gel-purified fragment is provided in TE or microinjection buffer (10mM Tris-HCl, pH 7.5, 0.1mM EDTA, 100mM NaCl) (Figure 3).

Figure 3. Example of purification of a 160 kb BAC fragment. BAC. Left Panel: BAC DNA digested with NotI, M1=Lambde ladder DNA marker, v=vector band ~7 kb. Right Panel:=LF: purified ~160-kb Large Fragment BAC, M2=supercoil DNA marker. Arrows indicate the large fragment BAC before and after purification.

Contact Lucigen for a free quote on any of our custom services.