EconoTaq® PLUS and EconoTaq PLUS GREEN 2X Master Mixes

EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR.

• More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes (Figure 1). High density reaction buffer enables direct gel loading following PCR.
• Excellent results in routine PCR. EconoTaq PLUS GREEN & EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb (Figure 1).
• Amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98°C to amplify challenging GC-rich templates others cannot (Figure 2).
• Convenient tracking dyes in EconoTaq PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp. See Figure 3. If desired, the dyes are easily removed by standard DNA purification products or ethanol precipitation. For fluorescence or absorbance detection assays, EconoTaq PLUS 2X Master Mix offers the same great performance without the dyes.
• Flexible & easy to use. EconoTaq PLUS GREEN and EconoTaq PLUS are packaged in 50 reaction vials that can be stored in the refrigerator (+4ºC) for up to 3 months. They are stable for at least 10 freeze-thaw cycles.
• Low price! EconoTaq PLUS GREEN is only 26¢ per 50 µl PCR reaction…for everything! EconoTaq PLUS GREEN & EconoTaq PLUS offer great value when compared to Taq master mixes.  EconoTaq PLUS GREEN & EconoTaq PLUS prices (see “Ordering and Pricing”) are based on a 50 µl PCR; using a 25 µl reaction volume will stretch your research budget even further.

Legal:

Please Note:
Some applications in which Lucigen's EconoTaq PLUS GREEN and EconoTaq PLUS 2X Master Mixes can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

PCR Activity: EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers.

Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl₂, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.

Absence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.

Purity: EconoTaq DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.The nucleotides in the Master Mix are certified free of nucleases and phosphatases.