DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus
- Recombinant DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus
- Multiple applications including DNA labeling and sequencing
Ordering
Recombinant Klenow Fragment, Exo- is supplied at a concentration of 5,000U/ml in a storage buffer of 50% glycerol, 25 mM Tris-HCl (pH 7.9), 0.1 mM EDTA and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.
Description
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus (Klenow Fragment, Exo-) is a DNA-dependent DNA polymerase which catalyzes the 5´→3´synthesis of DNA and lacks both the 5´→3´ and the 3´→5´ exonuclease activities.
Source:
Purified from a strain of E. coli that carries a Klenow Fragment, Exo- expression plasmid.
Applications:
- Random primed labeling (1)
- DNA sequencing by the Sanger dideoxy method (2)
Heat inactivation: 70°C for 15 minutes
Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.
Additional Information:
- Klenow Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on many DNA fragments during 5´-overhangs fill-in reactions. This enzyme is not recommended for preparation of blunt-ended fragments.
References:
1. Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.7-3.5.10.
2. Sanger, F. et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467.
Resources
| Document | File Name | Type |
| Manual | MA068_DNA_Polymerase_I(Klenow)_Fragment_Exonuclease_Minus | |
| MSDS |
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