No Size Limitation due to Packaging, Truly Unbiased Libraries
- Unbiased fosmid libraries.
- Larger inserts, fewer empty clones.
- Generate more results, faster, for less money.
Lucigen offers Custom Fosmid Library Construction Services featuring our exclusive Random Shear technology and the high efficiency CopyRight® v2.0 pSMART FOS vector (Figure 1).
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| Figure 1. Random Shear Fosmid Libraries with example average insert sizes: 90~100 and ~50 kb. |
- True, unbiased Fosmid libraries. Lucigen's exclusive Random Shear Cloning Technology and CloneSmart® transcription-free vectors eliminate biases in DNA preparation and cloning. Clone gaps and sequencing gaps are practically eliminated. Lucigen's technology eliminates the potential problem of generating chimeric clones-a common problem with conventional fosmid methods.
- Largest Fosmid insert sizes available. The average insert size of Lucigen Fosmid Libraries is up to 90 kb with a minimal (~10 kb) window of variation (Figure 1). Conventional fosmid libraries have a limitation of an average insert size 40 kb.
- More Fosmid recombinants per nanogram of input DNA. Lucigen's vector preparation methods, high efficiency CopyRight v2.0 FOS vectors, and the E. cloni® FOS Replicator™ Cells substantially increase recombinant yields. Conventional fosmid cloning requires high concentration and microgram quantities of purified large-insert DNA for ligation and packaging; Lucigen's technologies use 100-fold less DNA.
- Easy production of Fosmid DNA. A simple, single-step copy number induction in the CopyRight v2.0 system ensures high yields of Fosmid DNA, resulting in a >95% success rate in Fosmid end-sequencing.
Contact Lucigen for a free quote on any of our custom services.
