Cloning Kits & Vectors

Lucigen has a wide variety of cloning systems, including novel transcription-free cloning vectors, that improve cloning yields, reduce cloning bias, and improve sequencing throughput. For cloning the most difficult sequences, the BigEasy® v2.0 Linear Cloning System offers unmatched stability. Most Lucigen cloning vectors are pre-cut, dephosphorylated, and ready-to-use.

Use the new eLucidator™ interactive guide to select the best cloning vector for your application.

Go directly to Vector Sequences

Also see Protein Expression.

General Cloning

CloneSmart® Cloning Kits (pSMART® Vectors) » Stably clone any insert in transcription-free, blunt vectors for general cloning. Choice of antibiotic resistance and copy number.

pEZ Seq™ Cloning Kits » Clone and obtain maximum DNA sequence information. Option for blue/white screening.

PCR Cloning

GC Cloning and Amplification (pSMART® GC Vectors) » Stably clone PCR products from proofreading or non-proofreading enzymes.

GC Cloning and Amplification (pGC™ Blue Vector) » Clone PCR products with optional blue/white screening; includes T7 & SP6 RNA polymerase promoters.

BigEasy® Long PCR Cloning Kit (pJAZZ® Vectors) » Clone any amplification product, even “unclonable” fragments, up to 30 kb in pJAZZ® Vectors configured for PCR cloning.

Difficult Cloning or BAC/Fosmid Cloning

BigEasy® v2.0 Linear Cloning System (pJAZZ® Vectors) » Clone the unclonable, including large or highly repetitive DNA, solely available from Lucigen.

CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC) » Efficiently clone any insert 200kb.

CopyRight® v2.0 Fosmid Cloning Kits (pSMART® FOS) » Efficiently clone any insert 35-45kb.

Specialty Kits & Vectors

pInvRecA Vector » Transiently convert host to RecA+ to enable P1 transduction.

pRANGER™-BTB » Express protein in a broad range of Gram negative strains.

pJW168 Vector » Perform in vivo genetics using the cre/lox sytem.

Accessory Kits for Cloning

DNATerminator® End Repair Kit » Repair sheared or restriction-digested DNA to create blunt, 5' phosphorylated ends.

PCRTerminator® End Repair Kit » Remove single-base overhang and 5' phosphates to PCR products.

CloneDirect™ Rapid Ligation Kit » Proceed from ligation reaction to transformation in minutes.

UltraClone™ DNA Ligation and Transformation Kits » Ligate and transform any vector.

Gel-Ready™ DNA Ladders » Bench-top stable markers — always ready to use.

Bst Adapter Fill-In Kit » Close gaps remaining after linker or adapter ligation.