- Complete BAC sequence provided quickly (1~2 weeks).
- Library of clones provided at no additional cost.
- All information kept confidential.
Lucigen offers full length insert sequencing of large-insert cosmid, fosmid, and BAC clones using shotgun subcloning. In this method, purified DNA from the cosmid, fosmid or BAC clone is randomly sheared, end-polished, size-selected, and subcloned into Lucigen’s exclusive pSMART® Vector, and transformed into E. cloni® 10G Competent Cells. The colonies of the shotgun library are robotically picked into 96-well plates for high-throughtput DNA preparation and Sanger sequencing. Our service includes large-insert DNA preparation (optional), shotgun subcloning, library construction, colony picking, overnight culturing, DNA preparation from clones, DNA sequencing at any scale from a single sample to a 96-well high-throughput processing.
Service Features
- Exclusive pSMART shotgun library construction
- pSMART GC or triple-A vector capable of cloning inserts up to 10kb (typically 2-4kb)
- 95% or higher frequency of clones with inserts
- No chimeras
- Construction of libraries in custom plasmid vector available
- Fast turnaround time (1~2 weeks)
What you get
At the completion of the project your DNA sequences are transmitted via secure internet connection or provided on appropriate computer media. We will provide the complete shotgun library at no additional cost.
Contact Lucigen for a free quote
