“Lucigen’s cells ROCK! Few, if any, companies or individual laboratories make cells with as high efficiencies.” - Dr. Douglas Crawford, University of Miami, Miami, FL

Custom Competent Cells

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BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
BAC-Optimized Replicator v2.0 Electrocompetent Cells  12 rxns (DUOs) 60210-1 $155.00
   24 rxns (DUOs) 60210-2 $271.00
E. cloni 10G BAC-Optimized Electrocompetent Cells  10 rxns (Fivers) 60215-1 $115.00
   25 rxns (Fivers) 60215-2 $263.00
Recovery Medium  8 × 12 ml 80026-1 $92.00
YT Agar Powder  5 packets 60025-1 $41.00
ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

Description

Lucigen offers two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 2 × 1010 cfu/µg pKanR DNA).  These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields.  BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.

E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning.  Manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts, 10G BAC-Optimized Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G BAC-Optimized Electrocompetent

>1 × 1010

YES

YES

YES
without IPTG induction

BAC-Optimized Replicator v2.0 Electrocompetent

>2 × 1010

YES

YES

YES
IPTG induction required

Specifications

Genotypes

Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene)

E. cloni 10G BAC-Optimized: F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA

Resources

Document File Name Type
Manual MA052-BAC-Optimized-Replicator-Cells PDF
Manual MA030-E-cloni-10-G-BAC-Optimized-Electro PDF
PDF MSDS PDF

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