BAC End Sequencing Services

BAC End Sequencing Services

  • Over 90% sequence success rate.
  • Over 700bp per read.
  • All information kept confidential.

Lucigen offers BAC end sequencing with universal primers to the BAC vector, or gene-specific primers (supplied by the client). Our service includes robotic colony picking, overnight bacterial cell culture, DNA extraction from clones, DNA sequencing at any scale from a single sample to 96- or 384-well high-throughput processing.

Service Features

  • Robotic colony picking and overnight culturing
  • Automated high-throughput DNA preparation
  • BAC end DNA sequencing with high success and long reads

Typical results for Lucigen’s BAC end sequencing service provide our clients with an average of at least 90% success and reads >700bp for both forward and reverse directions (Figure 1).

What you get

At the completion of the project your DNA sequences are transmitted via secure internet connection or provided on appropriate computer media.  We typically provide raw sequence data and/or auto-trimmed BAC end sequences in FAST/TX formats.

Example of BAC End Sequencing

Figure 1. Example of a BAC end sequence with read length over 800 bp.

 

Contact Lucigen for a free quote