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E. cloni® 5-alpha Chemically Competent Cells: E. cloni® 5-alpha Chemically Competent Cells

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
  • Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.

Ordering

Product Description Size Cat. No. List Price Quantity BUY
E. cloni® 5-alpha Chemically Competent Cells  12 rxns (DUOs) 60602-1 $99.00
   24 rxns (DUOs) 60602-2 $171.00
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

Description

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols.  E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required.  These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Red arrows Custom Competent Cells

Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.

Table 1. E. cloni Competent Cells for Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

5-alpha Chemically Competent

>1 × 108 cfu/µg DNA

NO

NO

YES
No IPTG induction required

Specifications

Genotype

  • E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Resources

Document File Name Type
Manual MA054-E-cloni-alpha-Chemically-Competent-Cells PDF
Product Flyer E-cloni-Cells-2011 PDF
Product Flyer Competent-Cell-Comparison-2011 PDF
PDF MSDS PDF

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