MC1061 F-Electrocompetent Cells
Product Details
ShowCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
MC1061 F-Electrocompetent Cells
Key features
Show- Perfect for antibody phage display or peptide phage display library creation
- The highest efficiency TG1 competent cells available
- Your only source for electrocompetent SS320 and ER2738 cells
- Amber suppressor and non-amber suppressor lines for maximum flexibility
- High efficiency MC1061 F- cells for cloning or control reactions
- Create larger libraries; speed discovery
- Bulk custom dispensing available with all of our phage display cells
Product information
MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
TG1 Electrocompetent |
>4 × 1010 |
YES |
NO |
YES |
|
SS320 (MC1061 F') Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
MC1061 F- Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
|
ER2738 Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
Genotype
- TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
- SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
- ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
- MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
SDS
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